Atto 565 maleimide

C₃₇H₃₇ClN₄O₁₀

733.16

MFCD04040675

NA.32

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

200

BioReagent

powder

λ_ex 563 nm; λ_em 592 nm in 0.1 M phosphate pH 7.0

corresponds for coupling to thiols
suitable for fluorescence

−20℃

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)